In this article we will discuss about the life cycle of phage M13. M13 uses the F pilus of E. coli to infect the cell. Protein pill located on the tip of M13 contacts the TolA protein located on the pilus of host cell. This interaction causes a conformational change in pVIlI from 100% alpha-helix to 85% alpha-helix resulting in [...]
The following points highlight the six main therapies of bacteriophages. The therapies are: 1. Collection 2. Mode of Action of Phage Preparations 3. Commercial Production of Phages 4. Prophylaxis and Treatment of Bacterial Infections 5. Characteristics of Bacterlophages 6. Bio-Safety. Therapy # 1. Collection: Local samples of bacteriophage can be collected from water which possibly contains high quantities of bacteria [...]
In this article we will discuss about the replication of ssDNA phage. I. Adsorption: The cell wall surface of E.coli contains specific receptor sites. For example, the receptor site of ØX114 present on outer membrane of cell wall of Salmonella typhimurium is a lipopolysaccharide. The phage gets adsorbed on bacterial cell surface through any one of 12 icosahedral vertices. The [...]