The below mentioned article provides a study note on culture media. Growth and morphogenesis of plant tissues in vitro are largely governed by the composition of the culture media. Although the basic requirements for culturing plant tissue is same but in practice there are some specific nutritional requirements for promoting optimal growth from different kinds of explants in case of [...]
The following four main steps of tissue culture techniques. The steps are: 1. Inoculation of Explant 2. Incubation of Culture 3. Sub-Culturing 4. Transplantation of the Regenerated Plant. Step # 1. Inoculation of Explant: Successful control of contamination largely depends upon the precautions taken to prevent the entry of microorganisms at the time of transferring the sterilised explants on the [...]
The following points highlight the top eight types of tissue culture. The types are: 1. Seed Culture 2. Embryo Culture 3. Meristem Culture 4. Bud Culture 5. Callus Culture 6. Cell Suspension Culture 7. Anther Culture 8. Protoplast Culture. Type # 1. Seed Culture: Seeds may be cultured in vitro to generate seedlings or plants. It is the best method [...]