In this article we will discuss about the procedure for cloning recombinant DNA. The first step in a gene cloning programme is to construct a recombinant DNA molecule containing a donor DNA segment in which a selected gene is located and a vector DNA. The donor may belong to any taxonomic group. The DNA of […]
Procedure for Cloning Recombinant DNA
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Restriction Enzymes (With Diagram) | Microbiology
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These are endonucleases which cleave double-stranded DNA molecules at specific sites having a sequence of generally four to six nucleotides. These sites are called restriction sites. A specific restriction enzyme cleaves DNA wherever the site occurs. The nucleotide sequence of the restriction site is specific for each of hundreds of restriction enzymes discovered so far […]
Applications of Recombinant DNA Technology (7 Applications)
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The following points highlight the top seven applications of recombinant DNA technology in molecular analysis of disease. The applications are: 1. Normal Gene Variations 2. Gene Variations Causing Disease 3. Point Mutations 4. Deletions, Insertions and Rearrangements of DNA 5. Pedigree Analysis 6. Restriction Fragment Length Polymorphism (RFLP) 7. Gene Therapy. Applications of Recombinant DNA […]
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