A genome library consists of a large collection of bacterial cultures, in each of which a different segment of the genomic DNA of an organism has been cloned by recombinant DNA technology, so that the entire collection represents its complete genome. The analogy between a library of books and a genome library is based on […]
Genome Library (With Diagram) | Microbiology
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Restriction Enzymes (With Diagram) | Microbiology
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These are endonucleases which cleave double-stranded DNA molecules at specific sites having a sequence of generally four to six nucleotides. These sites are called restriction sites. A specific restriction enzyme cleaves DNA wherever the site occurs. The nucleotide sequence of the restriction site is specific for each of hundreds of restriction enzymes discovered so far […]
Vectors Used for Cloning of Larger Molecules of DNA
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In this article we will discuss about the three main types of vectors used in cloning for larger molecules of DNA. The types of vectors are: 1. Plasmid Vectors 2. Bacteriophage Vectors 3. Cosmid Vectors. 1. Plasmid Vectors: Plasmids are the extra chromosomal genetic elements commonly found in bacteria and are mostly made of double-stranded […]
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