The following points highlight the top four methods used for maintenance and preservation of pure cultures. The methods are: 1. Refrigeration 2. Paraffin Method 3. Cryopreservation 4. Lyophilisation. Method # 1. Refrigeration: Pure cultures can be successfully stored at 0-4°C either in refrigerators or in cold-rooms. This method is applied for short duration (2-3 weeks […]