In this article we will discuss about the determination of LC50 and LD50 of toxicants. Determination of LC50: Different procedures are adopted depending on the size of the organisms subjected to a toxicant. Mosquito Larvae (Test Animal): Four 1000 ml glass beakers are half-filled with tap water/tank water. A measured amount of toxicant-liquid/solid—is thoroughly mixed with water in three beakers—1, [...]
In this article we will discuss about the water sample collection for physical and chemical analysis. I. Collection of Water Sample: A representative sampling is usually done by collecting samples from a number of places from different depths and mixing them together to get a composite sample. A Kemmerer, Friedinger or Forest type sampler is used for water collection. Bubbling [...]
In this article we will discuss about the dissection of different contents for vertebrates and invertebrates under microscope. I. Gut Contents: Preparation: Cut a small portion of the gut, contents of which is to be studied. Put it in a watch glass. Open it giving a longitudinal incision with a pair of fine scissors. Add a small amount of physiological [...]