The following points highlight the three types of hormones which regulates the growth and development of plants. The hormones are: 1. Auxins 2. Cytokinins 3. Gibberellins. Hormone # 1. Auxins: Both naturally occurring and synthetic auxins are available and are widely used in cultures for clonal propagation work. Naturally occurring auxin is indole acetic acid (IAA). Synthetic ones are 2, [...]
In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]
In this article we will discuss about:- 1. Anther Culture Technique 2. Microspore Culture 3. Diploitization 4. Anther Vs. Pollen Culture 5. Plant Regeneration 6. Characteristics of Microspore Derived Plants 7. Ontogeny of Androgenesis. The term haploid refers to those plants which contains gametophytic number of chromosomes. Anther/pollen culture mediated production of haploid plants approves the concept of totipotency. Guha [...]