In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This direct enzymatic amplification process is [...]
In this article we will discuss about the role of RNA in transcription regulation. In the early 1960s, Francois Jocob and Jaques Monod performed exemplary work pertaining to prokaryotic gene regulation. These experimental techniques and strategies were later extended to eukaryotic system. Although control of transcription between prokaryotic and eukaryotic genes shares substantial homology, eukaryotic control of system is highly [...]
Exploitation of plant viruses as transformation vectors by massive infection may be harmful and even deleterious to the target plants. It is still however able to express and produce foreign proteins. Plant viruses must exhibit some of the exemplary features before they are considered as vectors. They should extend their broader host-range, spread of seed transmission and carry additional copies [...]