The following are the some of the examples of Somaclonal Variation: 1. Sugarcane 2. Potato 3. Tobacco 4. Rice 5. Barley. 1. Sugarcane: Application of somaclonal variation in crop improvement programme was established in sugarcane. The Hawaian Sugar Planters Association Experimental Station recorded genetic variation among sugarcane plants regenerated from tissue culture. Variations were recorded based on cytogenetic, morphological and [...]
In this article we will discuss about the process of in situ hybridization in nucleic acids. The mammalian and other higher eukaryons system contains larger DNA molecule. Some times larger than 50,000 kb. Hence, non-conventional methods like gel electrophoresis is not feasible. The gene location on these larger molecules can be identified by in situ hybridization which can prove accurate [...]
In this article we will discuss about the process of T-DNA transfer. Once plant exudates activating expression of vir genes and Agrobacterium attach to the plant cells, the T-DNA region in the Ti plasmid undergo excision process to transfer into the plant cells. Excision process involves active participation of vir genes such as virD, virC and virE. Separation of single [...]