In this article we will discuss about the process of cybrids production. These are cytoplasmic hybrids. In cybrids, nucleus of one parent and cytoplasm of both the parents constitute cytoplasmic hybrids. More precisely, cybrids can be accomplished by fusing protoplast derived from one of the parent plants with enucleated protoplast of other. Cybrids can also be obtained through various other [...]
The following are the some of the examples of Somaclonal Variation: 1. Sugarcane 2. Potato 3. Tobacco 4. Rice 5. Barley. 1. Sugarcane: Application of somaclonal variation in crop improvement programme was established in sugarcane. The Hawaian Sugar Planters Association Experimental Station recorded genetic variation among sugarcane plants regenerated from tissue culture. Variations were recorded based on cytogenetic, morphological and [...]
In this article we will discuss about the process of in situ hybridization in nucleic acids. The mammalian and other higher eukaryons system contains larger DNA molecule. Some times larger than 50,000 kb. Hence, non-conventional methods like gel electrophoresis is not feasible. The gene location on these larger molecules can be identified by in situ hybridization which can prove accurate [...]