In the preparation of culture media, different stock solutions (ATOG) are made. Generally stock solutions of major elements are prepared by 10X final concentration whereas minor elements and vitamins are prepared by 1000X final concentration and stored in the freezer. Stock solutions: A – NH4NO3 B – KNO3 C – CaCl2.2H2O D – KH2PO4 E — Molybdenum CaCl2 KI F [...]
In this article we will discuss about the process of in situ hybridization in nucleic acids. The mammalian and other higher eukaryons system contains larger DNA molecule. Some times larger than 50,000 kb. Hence, non-conventional methods like gel electrophoresis is not feasible. The gene location on these larger molecules can be identified by in situ hybridization which can prove accurate [...]
In this article we will discuss about the process of T-DNA transfer. Once plant exudates activating expression of vir genes and Agrobacterium attach to the plant cells, the T-DNA region in the Ti plasmid undergo excision process to transfer into the plant cells. Excision process involves active participation of vir genes such as virD, virC and virE. Separation of single [...]