According to Murashige of California University, micro-propagation is distinctly divided into five stages. Stage 0 — Mother Plant Selection: Before initiation of micro-propagation, selection of suitable mother plant is crucial in the whole exercise of propagation. Generally, disease free mother explant is selected for the micro-propagation to reduce contamination of cultures. Certain growth parameters of mother plant can be improved [...]
The following points highlight the three types of nucleic acid probes. The probes are: 1. Oligonucleotide Probes 2. DNA Probes 3. RNA Probes. Type # 1. Oligonucleotide Probes: These are synthesized chemically as oligonucleotides based on the information available on the amino acid sequence of the protein of interest. These oligo nucleotides can be used as a probe the identification [...]
The sequence of a deoxyribonucleic acid DNA molecule can be elucidated using chemical or enzymatic methods. Chemical Method of DNA Sequencing: In 1997, Maxam and Gilbert of Harward University discovered this method. In this method, a DNA fragment to be sequenced is radiolabeled at one end of molecule (Fig. 13.15). After denaturation of DNA into single strand, bases are modified [...]