In this article we will discus about the organization of plant mitochondrial DNA. The mitochondrial genome of higher plants is the largest and complex among the eukaryotes. However, the plant mitochondrial genome does not contain many genes. The complex nature of plant mitochondrial genome might be due to the presence of many chloroplast sequences in the mtDNA of higher plants. [...]
Developments of promoters that are activated in response to a specific chemical are particularly desirable in plant transformation. Utility of chemically inducible promoters are increasingly relevant to the improvement of crops by genetic engineering. Besides, several biotechnological applications of chemically inducible promoters have been witnessed recently such as construction of a male sterile system, expression of transgene that interfere with [...]
A genomic library contains all the sequences present in the genome of an organism. In the construction of genomic libraries it is feasible to use vectors that could accommodate large size of inserts. The first step in the construction of genomic library is the isolation of genomic DNA, and entire DNA is subjected to restriction digestion. The fragmented DNA of [...]