In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: […]
Estimation of Amylase | Enzyme Activity
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Detection of Ash in Plants | Botany
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A number of inorganic elements are present in plants which also catalyse many enzymatic reactions. The existence of such inorganic elements in plants can be detected by the chemical tests of plant ash. Requirements for Detection of Ash: (a) Preparation of Ash Extract: For detection of various elements in plant ash viz., Ca, Mg, Fe, […]
Determination of Chlorophyll and Carotenoids | Plants
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In this article we will discuss about the tests for spectrophotometric determination of chlorophyll and carotenoids. Extraction of Chlorophyll and Carotenoids: Although chlorophyllous and carotenoid pigments may be extracted readily in organic solvents, for many algae, extraction is not complete unless the cells are ruptured. Therefore, filters should be placed with forceps into the base […]
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