In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 […]
Estimation of Ascorbic Acid | Enzyme Activity
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