In this article we will discuss about the colorimetric method for estimation of phosphorus in plants. Principle: When ammonium molybdate solution is added to a solution of phosphate containing conc. H2SO4 it produces a yellow crystalline precipitation of ammonium phospho-molybdate. Phospho-molybdate reacts with amino-naphthol-sulphonic acid and produces a molybdenum complex which forms a blue- coloured […]
Estimation of Phosphorus by Colorimetric Method | Plants
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Estimation of Peroxidase | Enzyme Activity
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In this article we will discuss about the tests for estimation of peroxidase in plants. Principle: The enzyme peroxidase catalyses the oxidation of substrate by oxygen generated from the decomposition of hydrogen peroxide: 2H2O2 → 2H2O + O2 Substrate + O2 → Oxidized substrate. Requirements: (a) Reagents: 1. Hydrogen peroxide (H2O2) — 0.2% 2. Catechol […]
Estimation of Free Fatty Acid
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In this article we will discuss about the tests for estimation of free fatty acid. Principle: The free fatty acid in an oil is estimated by titrating it against KOH in the presence of phenolphthalein indicator. The acid number is defined as the mg KOH required to neutralize the free fatty acids present in 1 […]
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