In this article we will discuss about the tests for the detection of alkaloids in plants with the help of experiment. The alkaloids are a group of basic, secondary plant substance, which usually possess a N-containing hetero-cycle. Their basic character is due to this feature. The alkaloids are mostly colourless, crystalline and non-volatile solids. They […]
Detection of Alkaloids | Plants
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Detection of Enzymatic Activity | Plant Tissue
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Enzymes are thermo-labile organic catalysts which are very much sensitive to heat and pH. The activity of a number of respiratory enzymes could be detected within plant tissue by suitable tests. (a) Detection of Dehydrogenase: This enzyme accomplishes intracellular oxidation and reduction by the transfer of hydrogen from one type of molecule to another. The […]
Estimation of Proline | Plants
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In this article we will discuss about the tests for estimation of proline in plants. Principle: During selective extraction with aqueous sulphosalicylic acid, proteins are precipitated as a complex. Other interfering materials are also presumably removed by absorption to the protein-sulphosalicylic acid complex. The extracted proline is made to react with ninhydrin in acidic conditions […]
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