The replication of numerous procaryotes was studied in recent years. The mechanisms of the replication of phages T4, T7 and λ are now well known, while those of B. subtilis are beginning to be understood. However, the most extensively studied system remains Escherichia coli, thanks to the combination of various types of approaches. Experimental Approaches […]
Replication of DNA in E.Coli | Biochemistry
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Purine and Pyrimidine Deoxyribonucleosides | Biochemistry
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In this article we will discuss about the formation of purine and pyrimidine deoxyribonucleosies. Formation of Purine Deoxyribonucleosides-5′- Triphosphates: The deoxyribonucleotides are simply formed by reduction of the corresponding ribonucleotides. Thus reduction of carbon 2′ of ribose, takes place in E.coli and animal tissues at the ribonucleosides-5′-diphosphates stage, at the cost of a protein of […]
Biosynthesis of DNA | Biochemistry
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The biosynthesis of the DNA must consist in a replication or duplication. The study of the DNA structure as proposed by Crick and Watson (see fig. 6-15) gives an idea of the mechanism of this replication. Each nucleotide of the template DNA chain need only be recognized by a complementary nucleotide before the latter is […]
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