In this article we will discuss about SIRPH – A Rapid, Quantitative SNuPE-IP RP HPLC Method for Simultaneous Methylation Analysis at Multiple CpG Sites. Introduction: Bisulfite Based DNA Methylation Analysis: The most widely used protocols for detailed DNA methylation analyses are based on the use of bisulfite converted genomic DNA. This methodology was introduced by […]
DNA Methylation Analysis by DHPLC | Genetics | Biology
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Methods of DNA Sequencing | Nucleic Acids
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The sequence of a deoxyribonucleic acid DNA molecule can be elucidated using chemical or enzymatic methods. Chemical Method of DNA Sequencing: In 1997, Maxam and Gilbert of Harward University discovered this method. In this method, a DNA fragment to be sequenced is radiolabeled at one end of molecule (Fig. 13.15). After denaturation of DNA into […]
Types of Gel Electrophoresis | Nucleic Acids
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The following points highlight the two types of gel electrophoresis. The types are: 1. Polyacrylamide Gel Electrophoresis 2. Agarose Gel Electrophoresis Gel electrophoresis is the novel technique in which nucleic acid (even proteins) molecules are separated based on the size differences when subjected to electric field. In electrophoresis, the rate of migration in electric field […]
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