In this article we will discuss about the Extraction and Estimation of Deoxyribonucleic Acid (DNA). Principle: Extraction of DNA is accomplished by the rupturing of cell wall and nuclear membrane followed by deproteinization and precipitation of the nucleic acid using ethanol. Materials: a. Sample Material b. Extraction Medium 0-15 M NaCl, 0-1 M Na2 c. […]
Extraction and Estimation of Deoxyribonucleic Acid (DNA)
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Extraction and Estimation of Ribonucleic Acid (RNA)
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In this article we will discuss about the Extraction and Estimation of Ribonucleic Acid (RNA). Principle: The ribonucleoprotein complex is dissociated by SDS into RNA and protein, deproteinized by phenol and the free RNA left in aqueous solution is precipitated in the cold alter adding alcohol. Materials: a. Centrifuge (bench top) b Magnetic stirrer c. […]
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