In this article we will discuss about the isolation and sequencing of heteroduplex fragments from DHPLC analysis of somatic, low-frequency mutations. Introduction to Isolation and Sequencing of Mutations by DHPLC: Identification of somatic mutations in samples from solid tumors represent some additional challenges compared to the identification of heterozygous germline mutations. Biopsies from solid tumors […]
Isolation and Sequencing of Mutations by DHPLC | Genetics | Biology
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Types of Mutagens: Radiation and Chemical | Genetics
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Two types of mutagens are considered here which are mostly affected the humans producing different mutations resulting in a number of abnormalities: 1. Radiation Exposure 2. Chemical Mutagenesis. Type # 1. Radiation Exposure: High energy radiation or ionising radiation produces a genetic alteration or mutation at a very low dose also. It has been shown […]
DNA Repair Mechanisms Studied in Eukaryotes | Genetics
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In this article we will discuss about the five main DNA repair mechanisms that are studied in eukaryotes. The mechanisms are: 1. Photo-Reactivation or Photo-Repair 2. Dark or Excision Repair 3. Nucleotide Excision Repair 4. Mismatch Repair 5. Post-Replication Repair. Mechanism # 1. Photo-Reactivation or Photo-Repair: This mechanism was discovered when bacteria that had been […]
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