One of the most attractive features of light microscopy is the ease with which most specimens can be prepared for examination. Preparation often involves nothing more than mounting a small piece of the specimen in a suitable liquid on a glass slide and covering it with a glass coverslip. The slide is then positioned on […]
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Centrifugation and Subcellular Fractionation of Microscopic Components
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Centrifugation is a useful means of isolating and purifying cellular components because most organelles and macromolecules differ from one another significantly in size and density (and sometimes also in shape, especially in the case of macromolecules). These properties, as well as the density and viscosity of the surrounding medium, determine the rate at which a […]
Microscopy: Top 19 Things to Know About | Microbiology
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Everything you need to know about Microscopy. Some of the most frequently asked questions are as follows:- . Q.1. What is a Micrometer (µm)? Ans. A micrometer, also called a micron (µ) is equal to 10-6 m, i.e., 1/1000th mm. Q.2. What is a Nanometer (nm)? Ans. It was formerly called millimicron (mµ) and is […]