The culture media (nutrients) consist of chemicals which support the growth of culture or microorganisms. Microbes can use the nutrients of culture media as their food is necessary for cultivating them in vitro. Types of Culture Media: The first medium prepared was meat-infusion broth. As most pathogenic microbes require complex food similar in composition to the fluids of the animal [...]
The following points highlight the six main techniques which are used to develop for the isolation of pure culture from mixed culture. The techniques are: 1. Use of Micromanipulator 2. Isolation by Exposure to Air 3. Isolation by Streaking or Streak Plate Technique 4. Isolation by Inoculating in Animals 5. Isolation by Using Selective or Enrichment Media 6. Other Methods [...]
Growth is defined as an orderly increase in cellular components. Microorganisms grow in a variety of physical and chemical environments. To distinguish orderly growth with that of in orderly growth, in recent years the term "balanced growth" has been used. "Balanced growth" as defined by Campbell, is doubling of every biochemical unit of the cell within the time duration of [...]