Some important antibiotics which inhibit the protein synthesis in prokaryotes along with the reason are given below:- 1. Streptomycin: Because it binds to the S12 protein of the 30 S ribosomal subunit and therefore, inhibits binding of tRNA to the P site. It also causes misreading in a system that is in the 'act of synthesis. 2. Neomycin, Kanamycin: Both [...]
The important techniques involved in the study of molecular biology of proteins are:- 1. Purification from Cell Extracts 2. Using Column Chromatography 3. Affinity Chromatography 4. Separation of Proteins on Polyacrylamide Gels 5. Antibodies can be Visualized Electrophoretically: Separated Proteins 6. Sequencing Protein Molecules. Technique # 1. Purification from Cell Extracts: Purification of proteins is a major part of understanding [...]
The techniques used to study molecular biology of nucleic acids are:- 1. Gel electrophoresis 2. Use of restriction endonucleases 3. DNA hybridization 4. Hybridization probes 5. Isolation of specific segments of DNA 6. DNA cloning 7. Cloning DNA in plasmid vectors 8. Vector DNA can be introduced into host organisms by transformation 9. Libraries of DNA molecules can be created [...]