The following points highlight the top three staining methods used for colouring the biological materials. The types are: 1. Negative Staining 2. Simple Staining 3. Differential Staining. Method # 1. Negative Staining: For quicker observation of the morphology of bacterial or yeast cells, the negative staining technique is applied. Requirements: 1. Bacterial/yeast suspension. 2. Slide, inoculating needle, pipettes etc. 3. [...]
The following points highlight the two techniques applied for the preparation of specimen for microscopic examination. The techniques are: 1. Hanging Drop Technique 2. Fixed and Stained Smears. Technique # 1. Hanging Drop Technique: Hanging drop or wet preparations permit examination of organisms in a normal living condition. A wet mount is made by placing a drop of fluid containing [...]
In this article we will discuss about the Isolation of Various Microorganisms:- 1. Isolation of Algae, Fungi, Bacteria and Actinomycetes from Soil 2. Isolation of Bacteria from Milk Samples by Agar Plate Method 3. Isolation of Microorganisms from Air by Exposure Plate Technique 4. Isolation of Microorganisms from Water or Waste Water Samples 5. Isolation of Root Nodule Bacteria. Contents: [...]