The following points highlight the top four techniques of sterilisation which are employed to sterilize different materials needed for microbiological work. The techniques are: 1. Sterilisation by Heat 2. Sterilisation by Chemical Treatment 3. Sterilisation by Radiation 4. Sterilisation by Filtration. Technique # 1. Sterilisation by Heat: Heat is the most widely used lethal agent for sterilisation. Objects may be [...]
The following points highlight the top three staining methods used for colouring the biological materials. The types are: 1. Negative Staining 2. Simple Staining 3. Differential Staining. Method # 1. Negative Staining: For quicker observation of the morphology of bacterial or yeast cells, the negative staining technique is applied. Requirements: 1. Bacterial/yeast suspension. 2. Slide, inoculating needle, pipettes etc. 3. [...]
The following points highlight the two techniques applied for the preparation of specimen for microscopic examination. The techniques are: 1. Hanging Drop Technique 2. Fixed and Stained Smears. Technique # 1. Hanging Drop Technique: Hanging drop or wet preparations permit examination of organisms in a normal living condition. A wet mount is made by placing a drop of fluid containing [...]