The term electrophoresis describes the migration of a charged particle under the influence of an electrical field. Many important biomolecules — such as peptide, proteins nucleotides and nucleic acids — possess ionisable groups and, therefore, at any given pH, exist in solution as electrically charged species either as cations (+) or anions (-). Under the […]
Electrophoresis (With Diagram)
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Colorimetry: Principle and Instruments
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In this article we will discuss about the principle and instruments of colorimetry. Principle of Colorimetry: Colorimetry is a widely used technique applied in biological system. It involves the measurement of a compound or a group of compounds present in a complex mixture. The property of colorimetric analyses is to determine the intensity or concentration […]
6 Full Proof Techniques of Chromatography
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The following points highlight the six full proof techniques of chromatography. The techniques are: 1. Partition Chromatography 2. Ion Exchange Chromatography 3. Adsorption Chromatography 4. Gel Permeation Chromatography 5. Gas Chromatography Principle 6. High Performance Liquid Chromatography (HPLC). Technique # 1. Partition Chromatography: Two immiscible liquid phases are passed through the supporting phase-containing materials. This […]
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