The following points highlight the top eight methodologies necessary for genetic research. The methodologies are:- 1. Isolation of Plasmids 2. Isolation of Chromosomal DNA 3. Agarose Gel Electrophoresis 4. Restriction and Ligation 5. Transformation 6. Polyacrylamide Gel Electrophoresis 7. Two-Dimensional Electrophoresis and 8. Immunoelectrophoresis. Methodology # 1. Isolation of Plasmids: Plasmids are self-replicating, extrachro-mosomal genetic […]
Top 8 Methodologies Necessary for Genetic Research
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Structure of Lac Operon in E Coli | Genetics
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In this article we will discuss about the structure of lac operon in E. coli. The lac operon comprises three structural genes (z, y & a) and produces a polycistronic mRNA (Lac mRNA). This messenger RNA codes for β-galactosidase, lac permease and transacetylase. The lac permease is a single unit protein that acts as a […]
Structural Features of DNA | Genetics
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In this article we will discuss about some of the important structural features of DNA. 1. DNA exists as a double helix in which the two polynucleotide chains are coiled about one another in a spiral with a diameter of about 20Å (Fig. 1). 2. Concentration of thymine was always equal to the concentration of […]
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