Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control […]
Precision of Transgene Integration | Genetics
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Polymerase Chain Reaction (PCR) | Genetics
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In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This […]
Plant Viruses as Vectors | Genetics
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Exploitation of plant viruses as transformation vectors by massive infection may be harmful and even deleterious to the target plants. It is still however able to express and produce foreign proteins. Plant viruses must exhibit some of the exemplary features before they are considered as vectors. They should extend their broader host-range, spread of seed […]
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