Genetics | Biology Discussion

Methods to Create Marker Gene-Free Transformed Plants | Genetics

Four main methods have been suggested to create marker gene-free transformed plants. The methods are: 1. Co-Transformation 2. Site-Specific Recombination 3. Marker Gene Elimination by Transposase 4. Marker Gene Elimination by Intrachromosomal Recombination. Method # 1. Co-Transformation: Co-transformation strategies are popularly employed for the production of transformed plants free from marker gene(s). In this novel approach, gene of interest and [...]

By Garima G|2016-12-23T06:34:56+00:00December 23, 2016|Genetics|Comments Off

Precision of Transgene Integration | Genetics

Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control over the impact of genomic [...]

By Garima G|2016-12-23T06:35:55+00:00December 23, 2016|Genetics|Comments Off

Polymerase Chain Reaction (PCR) | Genetics

In this article we will discuss about:- 1. Meaning of PCR 2. Steps of PCR 3. Applications of PCR. Meaning of PCR: In genetic engineering, requirement of desirable amount of target DNA is indispensable to study genetic structure, function and gene manipulation. It is novel cell free techniques to obtain innumerable number of copies. This direct enzymatic amplification process is [...]

By Garima G|2016-12-23T06:34:56+00:00December 23, 2016|Polymerase Chain Reaction|Comments Off

Tag Archives | Genetics

Methods to Create Marker Gene-Free Transformed Plants | Genetics

Precision of Transgene Integration | Genetics

Polymerase Chain Reaction (PCR) | Genetics