Gene transfer in cereals is becoming established at the backdrop of recombinant DNA technology. Until recently, gene transfer in plants was accomplished only through certain conventional methods like sexual hybridization. The entire scenario of transformation was changed after the advancement of genetic engineering. Utility of Agrobacterium tumeifaciens as biological vector was found to be successful among dicots. However, its potential [...]
Four main methods have been suggested to create marker gene-free transformed plants. The methods are: 1. Co-Transformation 2. Site-Specific Recombination 3. Marker Gene Elimination by Transposase 4. Marker Gene Elimination by Intrachromosomal Recombination. Method # 1. Co-Transformation: Co-transformation strategies are popularly employed for the production of transformed plants free from marker gene(s). In this novel approach, gene of interest and [...]
Precision of Transgene Integration by Site-Specific Recombination ! Possibility of site-specific recombination and homologous recombination process has been exploited in recent days to potentially increase transgene integration. It has been developed into the genetic engineering tool for higher eukaryotes. Despite the efficiency of using Agrobacterium for transformation, integration is still random and has no control over the impact of genomic [...]