Depending on the nature of selection, marker genes are divided into two types: 1. Selectable Marker Genes 2. Reporter Genes. Type # 1. Selectable Marker Genes: One of the constraints in plant transformation technique is the obtaining of transformed plants with less frequency. Thus, it is indispensable to select transformed cells under cultural conditions itself. Selection is based on the [...]
Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]
In this article we will discuss about:- 1. Subject Matter of CamV35S Promoter 2. Molecular Dissection of CamV35S Promoter 3. Functional Regions. Subject Matter of CamV35S Promoter: The 35S promoter is one of the major promoters of cauliflower mosaic virus (CamV). This virus infects crucifers and contains double-stranded genome as the genetic material. Based on the 8 kb genetic map, [...]