Depending on the nature of selection, marker genes are divided into two types: 1. Selectable Marker Genes 2. Reporter Genes. Type # 1. Selectable Marker Genes: One of the constraints in plant transformation technique is the obtaining of transformed plants with less frequency. Thus, it is indispensable to select transformed cells under cultural conditions itself. Selection is based on the [...]
One of the significant developments in agriculture biotechnology is the successful transformation of plants by Agrobacterium as a vector. This has formed a basis for the better performance of plants. Existence of close proximity between plant and Agrobacterium leads to the exploitation of these bacteria for the production of transgenic plants. In addition, accumulation of substantial amount of information regarding [...]
Transformation of plants can be successfully accomplished by employing two basic strategies. One is co-cultivation of protoplast with Agrobacterium and the leaf disc procedure. The first transgene was obtained by co-cultivation. Basic methodology involves Agrobacterium transformation of regenerating protoplast, plus screening and regeneration. Leaf-disc transformation is used extensively in most of the transformation work. Surface- sterilized leaf disc or pieces [...]