Depending on the nature of selection, marker genes are divided into two types: 1. Selectable Marker Genes 2. Reporter Genes. Type # 1. Selectable Marker Genes: One of the constraints in plant transformation technique is the obtaining of transformed plants with less frequency. Thus, it is indispensable to select transformed cells under cultural conditions itself. Selection is based on the [...]
In this article we will discuss about:- 1. Subject Matter of CamV35S Promoter 2. Molecular Dissection of CamV35S Promoter 3. Functional Regions. Subject Matter of CamV35S Promoter: The 35S promoter is one of the major promoters of cauliflower mosaic virus (CamV). This virus infects crucifers and contains double-stranded genome as the genetic material. Based on the 8 kb genetic map, [...]
Developments of promoters that are activated in response to a specific chemical are particularly desirable in plant transformation. Utility of chemically inducible promoters are increasingly relevant to the improvement of crops by genetic engineering. Besides, several biotechnological applications of chemically inducible promoters have been witnessed recently such as construction of a male sterile system, expression of transgene that interfere with [...]