In this article we will discuss about: 1. Process of Somatic Embryogenesis 2. Embryo Maturation and Synchronisation 3. Cultural Conditions 4. Recurrent Embryogenesis and Mass Production 5. Applications. After fertilization, zygote is transformed into adult status through a series of embryogenic processes. Despite the same genetic constituents, somatic cells on the other hand, do not reorient towards embryo production. However, [...]
This article provides a case study on transgenic rice for stress tolerance. Production of agronomically useful transgenic rice has been given top priority in the improvement of crop plants. The loss of rice yield due to stress impact is estimated to cost at least several billion dollars a year. Genetic engineering offers effective way to deal with these problems. Moreover, [...]
Blotting technique is an extremely powerful tool for analyzing gene structure and used to study gene expression, once cloned cDNA is isolated. There are three important types of blotting techniques are: 1. Southern Blotting 2. Northern Blotting 3. Western Blotting. Technique # 1. Southern Blotting: Developed by E.M. Southern, the technique of Southern blotting is one of the most important [...]