Depending on the nature of selection, marker genes are divided into two types: 1. Selectable Marker Genes 2. Reporter Genes. Type # 1. Selectable Marker Genes: One of the constraints in plant transformation technique is the obtaining of transformed plants with less frequency. Thus, it is indispensable to select transformed cells under cultural conditions itself. Selection is based on the [...]
Gene transfer in cereals is becoming established at the backdrop of recombinant DNA technology. Until recently, gene transfer in plants was accomplished only through certain conventional methods like sexual hybridization. The entire scenario of transformation was changed after the advancement of genetic engineering. Utility of Agrobacterium tumeifaciens as biological vector was found to be successful among dicots. However, its potential [...]
Four main methods have been suggested to create marker gene-free transformed plants. The methods are: 1. Co-Transformation 2. Site-Specific Recombination 3. Marker Gene Elimination by Transposase 4. Marker Gene Elimination by Intrachromosomal Recombination. Method # 1. Co-Transformation: Co-transformation strategies are popularly employed for the production of transformed plants free from marker gene(s). In this novel approach, gene of interest and [...]