In this article we will discuss about the significance of haploids. Application in Crop Improvement: Productions of homozygous pure lines are the major goal in breeding strategy. These are obtained by back crossing and inbreeding. These processes are however, are time consuming. Once established, homozygous lines have potential for plant improvement in several ways. With the advent of anther culture, [...]
Blotting technique is an extremely powerful tool for analyzing gene structure and used to study gene expression, once cloned cDNA is isolated. There are three important types of blotting techniques are: 1. Southern Blotting 2. Northern Blotting 3. Western Blotting. Technique # 1. Southern Blotting: Developed by E.M. Southern, the technique of Southern blotting is one of the most important [...]
One of the significant developments in agriculture biotechnology is the successful transformation of plants by Agrobacterium as a vector. This has formed a basis for the better performance of plants. Existence of close proximity between plant and Agrobacterium leads to the exploitation of these bacteria for the production of transgenic plants. In addition, accumulation of substantial amount of information regarding [...]