In this article we will discuss about the novel DHPLC based strategy for identification and characterisation of (BI)-Clonal T-Cell receptor targets for the detection of minimal residual disease in childhood acute lymphoblastic leukemia. Introduction: Clonal antigen receptor rearrangements are widely used as patient-specific markers for detection of minimal residual disease (MRD) in acute lymphoblastic Leukemia […]
Novel and Innovative Applications of DHPLC | Genetics | Biology
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DNA Methylation Analysis by DHPLC | Genetics | Biology
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In this article we will discuss about SIRPH – A Rapid, Quantitative SNuPE-IP RP HPLC Method for Simultaneous Methylation Analysis at Multiple CpG Sites. Introduction: Bisulfite Based DNA Methylation Analysis: The most widely used protocols for detailed DNA methylation analyses are based on the use of bisulfite converted genomic DNA. This methodology was introduced by […]
Analysis of Promoter Methylation | DHPLC | Genetics | Biology
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In this article we will discuss about the analysis of promoter methylation using Denaturing High-Performance Liquid Chromatography (DHPLC). Introduction to Analysis of Promoter Methylation using DHPLC: Sequencing and analysis of the human genome revealed the presence of only 30,000 to 40,000 protein-coding genes, a stark contrast to previous estimates of about 100,000 genes. While a […]
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