In this article we will discuss about the mutation detection in the Tuberous Sclerosis Genes TSC1 and TSC2 by DHPLC. Overview of Tuberous Sclerosis (TSC): TSC is a relatively variable clinical syndrome, in which several pathologic manifestations are highly specific for the disease. The cardinal feature is the cortical tuber, which is a distinctive form […]
DHPLC Analysis of TSC1 and TSC2 Genes | Genetics | Biology
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DNA Methylation Analysis by DHPLC | Genetics | Biology
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In this article we will discuss about SIRPH – A Rapid, Quantitative SNuPE-IP RP HPLC Method for Simultaneous Methylation Analysis at Multiple CpG Sites. Introduction: Bisulfite Based DNA Methylation Analysis: The most widely used protocols for detailed DNA methylation analyses are based on the use of bisulfite converted genomic DNA. This methodology was introduced by […]
Analysis of Promoter Methylation | DHPLC | Genetics | Biology
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In this article we will discuss about the analysis of promoter methylation using Denaturing High-Performance Liquid Chromatography (DHPLC). Introduction to Analysis of Promoter Methylation using DHPLC: Sequencing and analysis of the human genome revealed the presence of only 30,000 to 40,000 protein-coding genes, a stark contrast to previous estimates of about 100,000 genes. While a […]
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