In this article we will discuss about non-radioactive and radioactive procedures of hybridization. Non-Radioactive Hybridization Procedures: Nucleic acid hybridization can be detected by labeling the probe with a radioactive isotope or a non-radioactive isotope. Therefore, hybridization procedure can be of two types depending upon the type of label used to label the probe. Non-radioactive hybridization […]
In this article we will discuss about:- 1. Basic Considerations of Nucleic Acid Hybridization 2. Hybridization Probes 3. Labeling of Hybridization Probes. Basic Considerations of Nucleic Acid Hybridization: Nucleic acid hybridization is a basic tool in molecular genetics which exploits the ability of single stranded nucleic acid molecules to hybridize with complementary sequences to form […]
Polymerase Chain Reaction-Oligonucleotide Ligation Assay (PCR-OLA) is a method to diagnose hereditary diseases caused by mutation not affecting restriction endonuclease sites. This method combines Polymerase Chain Reaction with the Ligation Assay. PCR-OLA distinguishes between the ligation and the absence of ligation of two oligonucleotides. A single nucleotide mismatch (at the site of hybridized oligonucleotides) prevents […]
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