In this article we will discuss about vectors used in genetic engineering. Cloning Vector: By cloning, one can produce unlimited amounts of any particular fragment of DNA. In principle, the DNA isolated and cut pieces are introduced into a suitable host cell, usually a bacterium such as Escherichia coli, where it is replicated, as the […]
Vectors Used in Genetic Engineering | Biotechnology
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Genetic Engineering: Purpose and Basic Steps | Biotechnology
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In this article we will discuss about the purpose and basic steps of genetic engineering. Purpose of Genetic Engineering: The interest in genetic engineering principally is due to its varied applications (Fig. 22.1): 1. Production of varieties of plants having particular desirable characteristics (e.g., resistance or tolerance to disease, drought, ‘development of CMS line, etc.). […]
2 Main Types of Screening Techniques | Biotechnology
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The following points highlight the two main types of screening techniques. The types are: 1. Colony Hybridisation Technique 2. Blotting Techniques. Type # 1. Colony Hybridisation Technique: This technique has been developed by Gaustein and Hogness (1975). It requires the radioactively labelled DNA probe with a sequence complementary to at-least one part of the desired […]
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