The following points highlight the seven steps for identification of bacteria isolated from a specimen. The steps are: 1. Morphology and Staining 2. Cultural Characteristics 3. Biochemical Reactions 4. Antigenic Characters 5. Typing of Bacteria: Bacteriophage Sensitivity 6. Animal Pathogen City 7. Antibiotic Sensitivity. Identification of Bacteria: Step # 1. Morphology and Staining: Serve as preliminary criteria. The Gram stained [...]
In this article we will discuss about the Antibiotic Sensitivity Test of Bacteria. Subcultures of the test organisms are made in broth and incubated for 6-18 hours. Any density of inoculum may be used but in most cases the surfaces of cultures plates may be sown with broth containing approximately 100 million cells per ml. After drying the surface, the [...]
The following points highlight the top two types of serology for recognition of antigen and antibody. The types are: 1. Agglutination 2. Precipitation. Serology: Type # 1. Agglutination: (a) Widal Test (Fig. 8.1): In the routine laboratory diagnostic Widal reaction, the patient's serum is tested simultaneously with the antigen of each organism likely to be responsible for enteric fever in [...]