The following methods highlight the two important methods of gene transfer used in plants. The methods are: 1. Indirect Method 2. Direct Method. 1. Indirect Method: Transfer via Agrobacterium: The transformation of a plant can be carried out directly by using Agrobacterium spp. which is a common bacterium causing crown gall tumour in legumes. This […]
Methods of Gene Transfer used in Plants
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3 Main Ways for Confirming Gene Transfer
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The following points highlight the three main ways for confirming gene transfer. The ways are: 1. Marker and Reporter Gene 2. Southern Blotting or Southern Hybridization 3. Northern Hybridization. Way # 1.Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. It is, therefore, important that there should be some means […]
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