The below mentioned article includes a collection of seven experiments on enzyme activity. 1. Experiment to demonstrate the activity of enzymes: Requirements: Benzidine solution, razor, thin sections of actively growing root (or germinating seeds or germinating pollen grains), phosphate buffer, hydrogen peroxide (1%), ammonium chloride (5%), starch paste. Method, observations and results: 1. Cut thin […]
Experiments on Enzyme Activity | Biochemistry
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Practical Experiment on the Test of Plant Viruses
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The below mentioned article provides a practical experiment on the test of plant viruses. Requirements: 1. TMV infected tobacco plant. 2. Healthy tobacco plant of the same age as that of the infected plant. 3. Homogenizer. 4. Filtration apparatus. 5. K2HPO4 – 1 % or (0.057M). 6. Distilled water, Carborundrum powder, etc. 7. Pipette, brush […]
Practical Experiment on Culturing of Lambda (λ) Phage
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The below mentioned article provides a Practical Experiment on Culturing of Lambda (λ) Phage. Principle: Bacteriophage λ is cultured in E.coli cell suspension. The phage is allowed to harbour and multiply till the complete lysis of bacterial cells. Then the bacterial cells are killed with chloroform and the phage particles are separated by centrifugation. Materials: […]
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