Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take 4 clean microscopic slides and [...]
The below mentioned article includes a list of three experiments on permeability. 1. Experiment to study the rates of penetration (permeability) of various organic solvents into living epidermal cells: Requirements: Onion bulbs, slides, cover slip, microscope, water, stop watch, glucose, sucrose, glycerol, methyl alcohol, tertiary butyl alcohol (TBA). Method: 1. Take an onion bulb and peel off a large segment [...]
The below mentioned article includes a collection of seven experiments on enzyme activity. 1. Experiment to demonstrate the activity of enzymes: Requirements: Benzidine solution, razor, thin sections of actively growing root (or germinating seeds or germinating pollen grains), phosphate buffer, hydrogen peroxide (1%), ammonium chloride (5%), starch paste. Method, observations and results: 1. Cut thin sections of fast-growing roots or [...]