Object: To separate the chlorophyll pigments by Thin Layer Chromatography (T.L.C.). Requirements: Spinach leaves, slides, staining tube, acetone, benzene, mortar, pastel, Kieselgel G, drier, pencil, sand, distilled water and oven. Preparation of T.L.C. Slides: 1. Prepare a slurry of Kieselgel by suspending about 2 gm. of Kieselgel in 10 ml. of distilled water. 2. Take […]
Experiment on Thin Layer Chromatography | Botany
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