In this article we will discuss about the tests for estimation of amylase in plants. Principle: The reducing sugars produced by the action of α- and or β-amylase react with di-nitro-salicylic acid and reduce it to a brown coloured product, nitro-amino-salicylic acid. Materials: i. Sodium acetate buffer, 0.1 M pH 4.7 ii. Starch 1% Solution: […]
Estimation of Amylase | Enzyme Activity
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