In this article you will discuss about the tests for estimation of catalase in plants. Principle: The enzyme catalase is responsible for catalysing the decomposition of H2O into H2O and O2. The assay of enzyme depends on estimation of residual H2O2 by titration with KMnO4. Requirements: (a) Reagents: 1. H2O2 —0.2 volume 2. H2SO4—10% 3. […]
Estimation of Catalase | Enzyme Activity
Article Shared by 
Estimation of Cellulase | Enzyme Activity
Article Shared by
In this article we will discuss about the tests for estimation of cellulase in plants. Principle: Endo-β-1, 4 glucanase acts on carboxymethyl cellulose (CMC) and hydrolyses the β-1, 4 glycosidic bonds in a random manner. As a result, the viscosity of CMC solution is reduced. This is measured in an Ostwald viscometer. Materials: a. Citrate-phosphate […]
Estimation of Ascorbic Acid | Enzyme Activity
Article Shared by
In this article we will discuss about the tests for estimation of ascorbic acid in plants. Principle: Ascorbic acid oxidase acts on the substrate ascorbic acid to form dehydro-ascorbic acid. The residual ascorbic acid content is determined by titration using a dye solution (Dichlorophenol indophenol, an oxidation-reduction dye): Requirements: (a) Reagents: 1. Dye solution: 15 […]
This is a question and answer forum for students, teachers and general visitors for exchanging articles, answers and notes. Answer Now and help others.
Answer Now
Answer Now
Here's how it works:
- Anybody can ask a question
- Anybody can answer
- The best answers are voted up and rise to the top
Powered by WordPress. Designed by 