The cloning of single genes is usually best carried out using plasmids, since the insert will rarely be larger than about 2 kb. However, for cloning of larger pieces of DNA (e.g., during gene library construction) these plasmids are not suitable as larger inserts increase the plasmid size, making the transformation inefficient. Large DNA molecules […]
Bacteriophage Vectors for E.Coli | Genetics
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The Genetic Map of E. coli | Genetics
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In this article we will discuss about the genetic map of E.coli. Several methods have revealed that the genetic map of the main chromosome of E. coli is an un-branched circle with a circumference of 100 minutes (Fig. 17.5) which corresponds to the transfer time during conjugation. About 650 genes have been mapped. Some important […]
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