However, even after transformation, it is essential to confirm the integration of genes, for the success of transgenesis. The incorporation can be confirmed through (i) The use of markers, (ii) Southern blotting, (iii) Northern blotting, and (iv) Polymerase chain reaction. (i) Marker and Reporter Gene: Transformation in eukaryotic system is a very low frequency event. […]
Confirmation of Transferred Gene | Genetics
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