In this article we will discuss about the DNA extraction and amplification. Normally, CTAB (cetyl trimethyl ammonium bromide) method is used. However, SDS method is also common and is described as follows: 1. The leaves are washed with sterilised distilled water and blot dried and cut into small pieces with a sterilised blade. 2. 1g of leaf material is placed [...]
Most molecular markers fall into one of three basic categories of techniques that use either hybridization or are based on the polymerase chain reaction (PCR): Category # 1: Hybridization Based (non-PCR) Techniques: Category I includes restriction fragment length polymorphism (RFLP) analysis, where probes are hybridized to filters containing DNA which has been digested with restriction enzymes. The resultant fragments are [...]
In this article we will discuss about agarose gel electrophoresis. To know about quality and quantity of genomic DNA, agarose gel electrophoresis is performed on genomic and amplified DNA. Larger molecules migrate more slowly because of greater friction drag and they form their way through the pores of gel, less efficiently than smaller molecules. Range of separation in gel containing [...]