In this article we will discuss about:- 1. Isolation of DNA to be Cloned 2. Insertion of Foreign DNA Fragment into a Vector 3A. Transfer of Recombinant DNA into Bacterial Cell 3B. Cloning in Eukaryotes 4. Detection of Recombinant Clone. Gene Cloning Technique # 1. Isolation of DNA to be Cloned: The DNA of […]
4 Main Gene Cloning Techniques | Biotechnology
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Study Notes on Gene Sequencing | Genetics
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The below mentioned article provides a study note on gene sequencing. When a particular gene of interest or a DNA fragment is isolated, the sequencing of that piece of DNA becomes essential. The sequence of DNA refers to the order of nucleotide bases along its sugar phosphate backbone. No technique can determine the sequence of […]
Polymerase Chain Reaction (PCR): Biology Notes on PCR
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The below mentioned article provides a note on Polymerase Chain Reaction (PCR). Gene Amplification: Polymerase Chain Reaction (PCR): PCR provides a simple and ingenious method for exponential amplification of specific DNA sequences by in vitro DNA synthesis, i.e., this technique has made it possible to synthesize large quantities of DNA fragments without cloning it. Kary […]
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