In this article we will discuss about the purpose and basic steps of genetic engineering. Purpose of Genetic Engineering: The interest in genetic engineering principally is due to its varied applications (Fig. 22.1): 1. Production of varieties of plants having particular desirable characteristics (e.g., resistance or tolerance to disease, drought, ‘development of CMS line, etc.). […]
Genetic Engineering: Purpose and Basic Steps | Biotechnology
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2 Main Types of Screening Techniques | Biotechnology
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The following points highlight the two main types of screening techniques. The types are: 1. Colony Hybridisation Technique 2. Blotting Techniques. Type # 1. Colony Hybridisation Technique: This technique has been developed by Gaustein and Hogness (1975). It requires the radioactively labelled DNA probe with a sequence complementary to at-least one part of the desired […]
4 Main Gene Cloning Techniques | Biotechnology
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In this article we will discuss about:- 1. Isolation of DNA to be Cloned 2. Insertion of Foreign DNA Fragment into a Vector 3A. Transfer of Recombinant DNA into Bacterial Cell 3B. Cloning in Eukaryotes 4. Detection of Recombinant Clone. Gene Cloning Technique # 1. Isolation of DNA to be Cloned: The DNA of […]
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