The below mentioned article provides a note on anther culture. Explants used for anther culture is very critical as the anther lobes bearing the PMC (pollen mother cell) of correct divisional stage signify for being divided to form the callus mass or the direct haploid embryo. Young flower buds with immature anther lobes are surface sterilised and the stamens are [...]
In this article we will discuss about the process of androgenesis, explained with the help of suitable diagrams. A. In Vitro Pathways of Androgenesis: The anther or pollen normally starts undergoing in the pathway of androgenesis within 2 weeks and it takes about 5-8 weeks to obtain complete plantlets. Haploid plants are formed in two ways: (i) Direct Androgenesis: Embryos [...]
In this article we will discuss about the meaning and advantages of pollen culture. Meaning of Pollen Culture: Microspore or the immature pollen can be used as the explant to get the haploid plants directly. For pollen or microspore culture the flower buds are collected, surface sterilized and the anther lobes are dissected out from the flower buds as before. [...]