The synthesis of nucleic acids (DNAs and RNAs) from nucleosides-5'- triphosphates is characterized by the necessity of conserving the genetic information intact. The DNA is indeed the carrier of this information which is transmitted from generation to generation; this notion is now well established, but it was long believed that this information was carried by proteins. It would therefore be [...]
Glutamic acid, aspartic acid and their amides occupy an important place in the metabolism of nitrogenous compounds. We know that glutamic acid was the main entry point of ammonia into biological compounds and we will see that its metabolism is linked to that of several other amino acids; as for its amide, glutamine, it plays an important role in the [...]
In this article we will discuss about: 1. Methods of Cloning, 2. Cloning of a Specific Gene, 3. Cloning of a Specific Gene, 4. Expression of Cloned Genes, 5. Cloning of Genes in Eucaryotic Cells and 6. Precautions to be taken during Experiments of Genetic Recombination. Methods of Cloning: In the past few years, an increasing number of laboratories have [...]