The following points highlight the five main steps for aseptic manipulation. The steps are: 1. Sterilisation of the Culture Vessels and Instruments 2. Sterilisation of Nutrient Media 3. Sterilisation of Culture Rooms and Transfer-Area 4. Sterilisation of Explant 5. Aseptic Transfer of Explant/Sub-Culturing. Step # 1. Sterilisation of the Culture Vessels and Instruments: For sterilisation […]