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Estimation of Acid Phosphatase | Enzyme Activity

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In this article we will discuss about the tests for estimation of acid phosphatase in plants. Principle: The enzyme phosphatase hydrolyzes p-nitro-phenol phosphate. The released p-nitro-phenol is yellow in colour in alkaline medium and is measured at 405 nm. The optimum pH for acid and alkaline phosphatases are 5.3 and 10.5, respectively. Materials: (i) Sodium […]

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Estimation of Acid Phosphatase | Enzyme Activity

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