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Difference between Entamoeba histolytica and Entamoeba coli:
Difference # E. histolytica:
A. Trophozoite stage:
(i) Fairly abundant in amoebic dysentery stools.
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(ii) The size varies from 10-30µ
(iii) The outline shows finger like pseudopodia.
(iv) Actively motile in nature.
(v) Cytoplasm:
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Ectoplasm—prominent;
Endoplasm—finely granular with ingested RBCs
Vacuoles—scanty, spherical and defined.
Nucleus:
Nuclear membrane uniformly stained with chromatin; karyosome is central. In saline preparation the nucleus is indistinct and small.
B. Precystic stage:
The size varies from 5 to 15µ
C. Cystic stage:
(i) Size: 6-18µ
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(ii) Chromatid body present with rounded ends and they are bar like.
(iii) Usually one or four nuclei are seen.
(iv) Nucleus not visible when unstained.
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(v) Kidophilic vacuole—Prominent in uninucleate phase.
Difference # E. coli:
(i) Not abundant in stools.
(ii) 20-40 µ
(iii) Pseudopodia not prominent.
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(iv) Sluggishly motile.
(v) Ectoplasm—not prominent;
Endoplasm—coarsely granular loaded with bacteria, yeasts etc.
Vacuoles—often numerous, clefts and cracks are found.
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Nuclear membrane shows chromatin irregularly stained; karyosome eccentric. In saline preparation the nucleus is large and distinct.
12 to 17 µ
10 to 30 µ
Absent
Usually two or eight.
Visible when unstained.
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Largest in two—nucleate phase.
