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The following points highlight the three main methods of permanent slide preparations. The methods are: 1. Safranin-Fast Green Combination 2. Crystal Violet-Erythrosin Combination 3. Haematoxylin-Safranin Combination.
Method # 1. Safranin-Fast Green Combination:
Method # 2. Crystal Violet-Erythrosin Combination:
Use aqueous crystal violet stain in place of safranin and erythrosin in place of fast green in the method discussed above. Other details are same as in safranin- fast green combination mentioned above.
Method # 3. Haematoxylin-Safranin Combination:
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It is also similar with above-mentioned safranin-fast green combination except following differences:
(i) Use haemcitoxylin in place of safranin.
(ii) Wash with ammonia water and then thoroughly wash with tap water.
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(iii) Use safranin stain after 70% alcohol and destain with 70% alcohol and then use 90% alcohol and absolute alcohol.
Other details are same as in safranin-fast green combination mentioned above.
